Coding

Part:BBa_K5048046:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-22)


Neae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244


Design Notes

We take the N-terminal domain of intimin of enterohemorrhagic Escherichia coli as our surface display system in the adhesion module. This system has been proven to be effective and safe by papers.


Source

N-terminal domain of intimin of enterohemorrhagic Escherichia coli.


References

Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e616. doi:https://doi.org/10.1016/j.cell.2018.06.041